Advertisement

Shimadzu Scientific Instruments’ nSMOL (nano-surface and molecular orientation limited proteolysis) Antibody Bio-Analysis Kit is applicable to a variety of pharmaceutical antibodies. The proprietary technique enables selective proteolysis of the Fab region of monoclonal antibodies to dramatically improve the productivity and robustness of LCMS mAB bioanalysis. The kit is a ready-to-use reagent kit for collecting monoclonal antibodies from blood or other biological samples using immunoglobulin collection resin, and then performing selective proteolysis of the Fab region of these antibodies via trypsin-immobilized nanoparticles. Variable region-derived peptides produced by limited proteolysis can then be quantified via MRM measurements utilizing the company’s high-performance LCMS-8050/8060 triple quadrupole liquid chromatograph mass spectrometer. The simplified workflow eliminates the steps of denaturing, reduction, and alkyation normally associated with protein digestion, resulting in more efficient sample preparation and analysis.
Shimadzu Scientific Instruments, Inc.
www.ssi.shimadzu.com, 410-381-1227

Advertisement
Advertisement