Several organisms possess ion channels called channelrhodopsins, which can be switched on and off with the help of light. Different channelrhodopsins respond to different wavelengths in the light spectrum. These channels can be expressed in foreign organisms by means of genetic engineering, which in turn finds applications in optogenetics, the application of light to modulate cellular and gene functions. Previously, the shortest wavelength that a channelrhodopsin responded to was blue.
However, recently, a group of scientists from the Nagoya Institute of Technology, Japan, and Jawaharlal Nehru University, India, identified a channelrhodopsin that responds to an even shorter indigo blue wavelength of light. In their study published in Communications Biology, the group of researchers, led by Hideki Kandori and Satoshi Tsunoda, identified a novel channelrhodopsin, which they named KnChR, from a species of terrestrial alga called Klebsormidium nitens.
"We chose this alga, because it is known to be responsive to light, but its photoreceptor domain has not been established," said Kandori. Unlike other discovered channelrhodopsins, KnChR was found to respond to indigo blue ligh.
It is known that KnChR is made up of a seven-cell membrane spanning region, which forms the pore that allows the entry and exit of different ions. This region is followed by a protein moiety including a peptidoglycan binding domain. In order to investigate the properties of KnChR, the researchers performed extensive genetic and electrophysiological experiments.
What was perhaps the most exciting result was that they could identify the role of the cytoplasmic domain. All known channelrhodopsins have a large cytoplasmic domain, or region that is located in the internal area of the cell.
"All currently known channelrhodopsins comprise a large cytoplasmic domain, whose function is elusive. We found that the cytoplasmic domain of KnChR modulates the ion channel properties," said Kandori.
Accordingly, the results of the experiments showed that changing the lengths of the cytoplasmic domain caused the changes in ion channel closure. Particularly, the shortening of the domain resulted in increased channel "open time" by more than 10x. In addition, the researchers identified two arginine amino acid residues, R287 and R291, in the same region, which play an important role in the properties of generated light currents. They found that KnChR exhibited maximal sensitivity at 430 nm and 460 nm, making it the "bluest" channelrhodopsin.
Overall, the researchers have faith in the KnChR being helpful in biological systems requiring specific excitation parameters.
"KnChR would expand the optogenetics tool kit, especially for dual light applications when short-wavelength excitation is required," said Tsunoda, who is the corresponding author of the study.
What this means is that the light-operated property of KnChR can be applied in targeted manipulation of an organism's biological functions, in a research setting. A few examples would include manipulation of neuronal and myocyte activities. Indeed, the researchers hope that the scope of the discovery will expand beyond the laboratory into real-world applications. These real-world applications could include cure for Alzheimer's disease and heart diseases, light therapy for recovery from depression, and visual restoration.
Republished courtesy of Nagoya Institute of Technology. Photo: KnChR allows sodium ions and calcium ions to pass through in the presence of indigo light. Credit: Hideki Kandori/Nagoya Institute of Technology