Purified proteins are required for many proteomics applications such as X-ray crystallography, NMR, mass spec, and in vitro biochemical assays. Proteins can be isolated from tissue or, more often, by their overexpression in an organism, like bacteria, yeast, or mammalian cells in culture.
This application note focuses on two challenging areas for nanoparticles: reliable, rapid scale-...
Refractometers are used to continuously monitor the concentrations of liquids used in the food...
Exosomes purification and analyses comprise a fast evolving research area; more than 70% of...
Nuclear fragmentation is not widely employed to quantitatively assess apoptosis because of the small number of cells that can be examined under the microscope. A number of biochemical tests have, as a result, have been adopted in place of the analysis of nuclear fragmentation. However, visual evidence of nuclear fragmentation has historically been considered the ‘gold standard’ for identifying apoptotic cells. This white paper examines the best way to observe and quantify apoptosis by nuclear fragmentation.
Are you annoyed with inaccurate pipetting results? With these 10 simple tips, you will improve your results instantly! Learn: What to do before you start pipetting How to pipette How to optimize pipetting performance
Mobile Forensic Software is a very important tool for the forensic investigator community. An ever evolving stream of technology available to criminals today, calls for mobile forensic software to stay one step ahead of the game. This webinar explores the latest trends in Mobile Forensic software including the newest pin code, pass code and pattern breaking software for androids and Iphones.
The production of pharmaceutical products requires the analysis of a series of essential trace elements as well as elements that are toxic in larger concentrations. Aspects such as bio-availability, toxicity and quantity of the elements must be considered. The sources of such impurities can be production-related contamination. This white paper explores the source of these impurities as well as the best methods to analyze them.
Manufacturing efficiencies and other changes now offer the possibility of obtaining and ICP-OES (inductively coupled plasma optical emission) product at a similar cost to the AAS device. Many users previously locked into AAS(atomic absorption spectrometry)technology are now considering switching to ICP-OES for their next analytical instrument purchase. This white paper briefly outlines conventional flame AAS and ICP-OES technologies and offers comparisons to help users choose the analyzer that’s right for them.
The proper selection and installation of equipment for your carrier gas system will help to ensure that you obtain the best possible performance from your GC instrument. A properly installed system and operation will pay for itself in added column lifetime, minimum system downtime, and better analytical results. Remember, doing it right the first time is less expensive than doing it over.
Sterility testing of final drug products is a mandatory release test. Traditional methods such as membrane filtration require a minimum incubation time of 14 days. This extended time to result delays product release and increases storage costs. Download this white paper and learn how to achieve results in just 5 days!
This new application note demonstrates the utility of the Chromatrap® ChIP-seq kit to elucidate the genome-wide binding patterns of nuclear receptor binding events in human adenocarcinoma cells; highlighting the fast multiplex capability alongside massively parallel NGS.
Ion chromatography (IC) is evolving to meet the analytical demands for more rapid analyses using significantly smaller sample volumes. Sub-5 minute analysis times and sub-ul sample volumes are now becoming available for some applications, along with a number of improved detectors. To take full advantage of these changes it is essential that sensitivity and reproducibility are maintained. The purity of the water used has a key role to play.
Ion chromatography (IC) is a form of liquid chromatography that is used to separate atomic or molecular ions for qualitative or quantitative analysis. As scientists demand ever more rapid analyses with smaller sample volumes, ultrapure water plays a key role in maintaining the sensitivity and reproducibility of IC applications.
Thousands of scientists around the world are making use of cutting edge technologies every day in order to push the boundaries of what we thought possible, furthering our understanding of the world around us. This dedicated pursuit of knowledge rests upon the shoulders of advances in the development of analytical instrumentation.
In this report, apoptosis was induced in HL60 cells with daunorubicin, a DNA-intercalating agent which inhibits DNA and RNA synthesis and is used as a treatment for acute myeloid leukemia (AML). HL60 cells were exposed to a time course of daunorubucin treatment.
The ImageStream® imaging flow cytometry platform was used to study the mechanism of action of Rituximab (RTX). RTX is a therapeutic monoclonal antibody directed against CD20 for the treatment of Non-Hodgkin’s lymphoma.
Increasing the number of samples which can be run simultaneously is desirable for high content analysis and screening applications. To increase sample throughput, the fluorescent cell barcoding method previously reported by Krutzik & Nolan has been adapted for the ImageStream®.
Many assays for immune function require imaging, but immune cells present significant challenges to image-based analysis due to their rarity and the need for simultaneous multispectral immunophenotyping, making statistically robust quantification difficult.