Purified proteins are required for many proteomics applications such as X-ray crystallography, NMR, mass spec, and in vitro biochemical assays. Proteins can be isolated from tissue or, more often, by their overexpression in an organism, like bacteria, yeast, or mammalian cells in culture.
This application note focuses on two challenging areas for nanoparticles: reliable, rapid scale-...
Refractometers are used to continuously monitor the concentrations of liquids used in the food...
Exosomes purification and analyses comprise a fast evolving research area; more than 70% of...
Ion chromatography (IC) is evolving to meet the analytical demands for more rapid analyses using significantly smaller sample volumes. Sub-5 minute analysis times and sub-ul sample volumes are now becoming available for some applications, along with a number of improved detectors. To take full advantage of these changes it is essential that sensitivity and reproducibility are maintained. The purity of the water used has a key role to play.
Ion chromatography (IC) is a form of liquid chromatography that is used to separate atomic or molecular ions for qualitative or quantitative analysis. As scientists demand ever more rapid analyses with smaller sample volumes, ultrapure water plays a key role in maintaining the sensitivity and reproducibility of IC applications.
Thousands of scientists around the world are making use of cutting edge technologies every day in order to push the boundaries of what we thought possible, furthering our understanding of the world around us. This dedicated pursuit of knowledge rests upon the shoulders of advances in the development of analytical instrumentation.
In this report, apoptosis was induced in HL60 cells with daunorubicin, a DNA-intercalating agent which inhibits DNA and RNA synthesis and is used as a treatment for acute myeloid leukemia (AML). HL60 cells were exposed to a time course of daunorubucin treatment.
The ImageStream® imaging flow cytometry platform was used to study the mechanism of action of Rituximab (RTX). RTX is a therapeutic monoclonal antibody directed against CD20 for the treatment of Non-Hodgkin’s lymphoma.
Increasing the number of samples which can be run simultaneously is desirable for high content analysis and screening applications. To increase sample throughput, the fluorescent cell barcoding method previously reported by Krutzik & Nolan has been adapted for the ImageStream®.
Many assays for immune function require imaging, but immune cells present significant challenges to image-based analysis due to their rarity and the need for simultaneous multispectral immunophenotyping, making statistically robust quantification difficult.
Gold Nanorods (AuNR) hold great promise for biomedical imaging. AuNRs have very strong absorption peaks in the visible and near-infrared region due to a plasmonic effect; the aspect ratio of the AuNR directly determines the wavelength of the peak.
Shared facilities, or common equipment rooms shared by several individual laboratories, often result in a laboratory having a number of unique users with varying levels of centrifuge experience using floor-standing ultracentrifuges and high-performance centrifuges.
Floor-standing ultracentrifuges and high-performance centrifuges are vital to many bioproduction processes. Instruments are run in Good Manufacturing Practice (GMP) environments, and must support compliance with these regulations.
This whitepaper reviews the causes of the three major allergic reactions to single-use glove products in Life Sciences environments, their symptoms, and glove solutions that are available to help prevent adverse reactions.
ChIP is a commonly used immunprecipitation technique for mapping the DNA-protein interactions in cells which are crucial for correct gene regulation. In a ChIP assay DNA-protein complexes (Chromatin) are selectively immunoprecipitated using antibodies directed against the protein of interest and the resulting fractions treated to separate the DNA and protein components.
A cleaning validation method has been developed for MICRO 90 Concentrated Cleaning Solution using a swab recovery technique and quantification with HPLC with charged aerosol detection (CAD) and mass spectrometry (MS) detection. The recovery of the sampling method was determined using HPLC-CAD; limit of detection (LOD) and limit of quantification (LOQ) were obtained using HPLC-MS.
Explore the cleaning efficiency of MICRO-90®, MICRO® GREEN CLEAN and MICRO® A07 on four GE flat sheet membranes in the GE Osmonics SEPA CF II filtration unit. The four membranes include a Cellulosic NF polymer, a proprietary thin film, a polyethersulfone, and an Ultrafillic UF membrane. Download to see the full research report.
Based on the membrane foulants, Micro-90®, Micro® A07, or both may be required to clean and return the conditions to their original values. The foulant will help determine which cleaner(s) is needed. If one cleaner is all that is necessary to correct the condition, then that is satisfactory. Sometimes, a pre-soak is useful. In other systems, both an alkaline and acidic cleaner are used for full cleaning. The goal of filter membrane cleaning is to return the flux rate as close to 100% of the original as possible, as well as correct the other pressure and water conditions.