Purified proteins are required for many proteomics applications such as X-ray crystallography, NMR, mass spec, and in vitro biochemical assays. Proteins can be isolated from tissue or, more often, by their overexpression in an organism, like bacteria, yeast, or mammalian cells in culture.
This application note focuses on two challenging areas for nanoparticles: reliable, rapid scale-...
Refractometers are used to continuously monitor the concentrations of liquids used in the food...
Exosomes purification and analyses comprise a fast evolving research area; more than 70% of...
No matter how routine the analysis, care must be taken when preparing fresh titrants and standardizing them; calibrating and maintaining appropriate sensors and handling samples. This guide discusses critical factors that can affect titration results and provides insight into how to elimination some of the more common sources of titration errors.
This paper focuses specifically on how thermal expansion affects repeatability. While accuracy is also affected by temperature change, in most applications, it is less of a concern. An accuracy reduction of several micrometers will usually be overshadowed by other sources of error within the state. For the most precise position applications, the effect of thermal expansion on accuracy will indeed require close scrutiny.
What Makes Your Laboratory Unique? Your people, your protocols, the work you do to change the world – and the instruments and equipment that help you to reach your goals. You shouldn’t have to fit your workflows around standard equipment. Your Equipment should be adaptable to you, while operating within the safety, performance, and compliance parameters that your work requires. In short, your laboratory equipment should work the way you do.
A new breed of manual pipettes has emerged that, unlike traditional pipettes which utilized a single rotating plunger to set volumes, features a revolutionary approach to manual pipetting. This new class of manual pipettes utilize an adjustable dial system to help reduce repetitive stress injuries and allow the user to set volumes ten times faster. Coupled with unique plunger settings that permit customized plunger force, this new evolution in manual pipettes improves manual pipetting techniques tenfold.
This app note outlines an innovative method to statistically quantify and localize carbon nanotubes (CNTs) in cells. This state-of-the-art method can be adapted to a large range of experiments and more specifically could overcome the difficulties of discriminating graphene-based nanomaterials from carbon-rich cell structures.
New advances in multispectral imaging have combined the capabilities of microscopy and flow cytometry into a single platform that is used for quantitative image-based cellular assays in large and heterogeneous cell populations. These capabilities can be utilized to measure both BRDU staining and nuclear fragmentation in a TUNEL assay. These new advances will also allow for enhanced utility of TUNEL assays as well as the identification of apoptotic cells by their unique nuclear morphologies.
Sample preparation is a key part of the analytical process, contributing to reproducibility and accuracy in the final results. The evaporation/concentration step can be achieved with various technologies, including heat, vacuum and blow-down. This webinar will examine the parameters that go into each of these choices and describe criteria to consider in matching the sample to the technique.
As reaction components for real-time PCR experiments come in a range of different labware formats, scientists traditionally have used single channel pipettes to prepare the reaction mixes. Unfortunately preparing and transferring samples one by one is not only error prone but also very time consuming. In this technical note the authors compare the results from using an 8-channel VOYAGER pipette and a conventional single channel pipette to prepare eight samples of extracted human gDNA in twelve replicates for real-time PCR.
Maintaining brand reputation and product quality has led to increased focus on qualifying raw materials and ingredients used in food production. In addition to traditional quality parameters, detection of adulterants plays a critical role in ensuring food safety and quality. FT-NIR spectrometers have become valuable tools for screening almost any raw material for adulterants in an easy and non-destructive way.
The analysis of composite films is often very complicated. Due to the complexity of the multiple layers, microscopic examination is often essential. While conventional optical microscopes allow for differentiating between the various layers, they do not allow for identification of the respective materials. In contrast, FT-IR microscopy allows identifying different materials in composite films and determination of their distributions.
Nuclear fragmentation is not widely employed to quantitatively assess apoptosis because of the small number of cells that can be examined under the microscope. A number of biochemical tests have, as a result, have been adopted in place of the analysis of nuclear fragmentation. However, visual evidence of nuclear fragmentation has historically been considered the ‘gold standard’ for identifying apoptotic cells. This white paper examines the best way to observe and quantify apoptosis by nuclear fragmentation.
Are you annoyed with inaccurate pipetting results? With these 10 simple tips, you will improve your results instantly! Learn: What to do before you start pipetting How to pipette How to optimize pipetting performance
Mobile Forensic Software is a very important tool for the forensic investigator community. An ever evolving stream of technology available to criminals today, calls for mobile forensic software to stay one step ahead of the game. This webinar explores the latest trends in Mobile Forensic software including the newest pin code, pass code and pattern breaking software for androids and Iphones.
The production of pharmaceutical products requires the analysis of a series of essential trace elements as well as elements that are toxic in larger concentrations. Aspects such as bio-availability, toxicity and quantity of the elements must be considered. The sources of such impurities can be production-related contamination. This white paper explores the source of these impurities as well as the best methods to analyze them.
Manufacturing efficiencies and other changes now offer the possibility of obtaining and ICP-OES (inductively coupled plasma optical emission) product at a similar cost to the AAS device. Many users previously locked into AAS(atomic absorption spectrometry)technology are now considering switching to ICP-OES for their next analytical instrument purchase. This white paper briefly outlines conventional flame AAS and ICP-OES technologies and offers comparisons to help users choose the analyzer that’s right for them.