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Kit Improves RNA Extraction from Tissue The AMSBIO ExpressAr kit for the extraction of RNA from FFPE (formalin-fixed and paraffin-embedded) tissues can be applied not only to PCR but also to microarray applications. The kit is optimized for isolation of total RNA from archival, formaldehyde or FFPE tissues and results in a concentrated RNA product that is ready to use. Using special lysis and incubation conditions, the kit and mRNA amplification technology can extract high-quality RNA and amplify small amounts. The result is the optimal consistency of high-quality RNA, as determined by RNA yields, bioanalyzer RNA profiles, and real-time qPCR data. The kit requires no overnight lysis step—users can deparaffinize in the morning and perform the RT-qPCR, mRNA amplification or microarray analysis in the afternoon. Able to work with up to five sections (10 to 20 µm thick), or up to 30 mg of unsectioned core samples, per reaction, the kit results in typical yields of ~2-µg RNA per slide.
AMS Biotechnology (AMSBIO) 63B Milton Park, Abindgon Oxon OX14 4RX Phone: +44-1235-828200 Fax: +44-1235-820482 http://www.amsbio.com
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