Bio-Rad Laboratories’ Image Lab 4.1 software for use with the ChemiDoc MP imaging system automates multiplex analysis, providing a faster, more accurate alternative for quantifying protein expression levels via western blotting. The software automates total protein normalization of multiplex fluorescent western blots by extracting image data directly from the ChemiDoc MP imager. Traditional image analysis methods require researchers to extract data into a spreadsheet program and manually manipulate data to produce a result. With this software, however, researchers can deliver normalized intensities automatically by selecting detection and normalization channels, then clicking the “normalization” button. Normalization is available with chemiluminescent blots as well as multiplex fluorescent blots. Stain-free total protein normalization is part of the company’s V3 Western Workflow, which speeds the western blotting process and provides researchers with the reassurance that their immunoblotting process is working as expected. Using stain-free detection, scientists can visualize protein separation without staining, verify protein transfer to the membrane and validate the results by total protein normalization.