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HILIC Columns Retains Highly Polar Compounds

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Thermo Fisher Scientific Hypersil GOLD HILIC columns, available with 1.9-, 3- and 5-µm particle sizes, are suited for applications within drug discovery, food testing and environmental analysis. Hydrophilic interaction liquid chromatography (HILIC) is becoming an increasingly popular technique that offers complementary selectivity to reversed-phase. With the ability to retain highly polar and hydrophilic compounds, this HILIC range of columns has been developed to aid the analysis of compounds that are traditionally difficult to retain using conventional C18 columns.

HILIC works by incorporating water in the highly organic mobile phase to form, an adsorbed water-rich layer on the polar stationary phase surface into which analyte molecules partition. Retention is governed by dipole-dipole interactions and hydrogen bonding mechanisms. Furthermore, the highly organic mobile phase contains low salt levels, making the columns suitable for use with electrospray mass spectroscopy.

Thermo Fisher Scientific/Fluid Handling
28W092 Commercial Ave.
Barrington IL 60010
http://www.thermo.com/fluidhandling

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