May 2009
The drug discovery and biotechnology industries continually demand large numbers of cells and high quality biologicals with minimal investment. One by product of this is the movement of some cell culture growth being conducted in large warm rooms without atmospheric control. To achieve this, media designed with buffering systems suitable for atmospheric CO2 levels must be used.
We show here that in addition to its use in more standard CO2 dependent culture environments Corning’s HYPERFlask cell culture vessel can be used in CO2-independent environments. A comparison of cell yields and metabolite analyses from Vero, MDBK, and 5/9 alpha CHO cell lines in a variety of CO2-independent media demonstrates that there is no difference in growth dynamics in the HYPERFlask vessel as compared to a traditional plugged T-flask. The versatility of the HYPERFlask cell culture vessel enables large-scale cell yields and biological production in a variety of atmospheric environments without the constraint of limited incubator space.
Click here to view this entire PDF
