Application of a GPC-LC-MS/MS Method for the Determination of Various Mycotoxins in Edible OilsFebruary 2009 The use of gel permeation chromatography (GPC) for sample clean-up in mycotoxin analysis was first reported in 1989 by Hetmanski and Scudamore. They developed a method for the determination of aflatoxins in cereals and animal feed. Later, GPC-methods for zearalenone (ZEA), ochratoxin A and other mycotoxins in cereals and feed were published. For the clean-up of mycotoxins from edible oils and fatty matrices GPC is currently regarded to be a promising approach. Kappenstein et al. developed a method for ZEA in corn oils and reported levels up to 920 μg/kg. Also traces of T-2 toxin have been reported to occur in this matrix. All these GPC methods were based on commonly used BioBeads SX-3 which appeared to be suitable for ZEA and aflatoxins as well as trichothecenes. But the selectivity of this material is not adequate to separate fumonisins (FB1/FB2) from the oil fraction. However, in the first version of commissions regulation (EC) 1881/2006 from 19 december 2006, a maximum level of 1000 μg/kg had been proposed for these toxins in maize oil. Here a new-developed GPC-column is presented which has been designed by LCTech for the simultaneous clean-up of zearalenone, fumonisins, trichothecenes (type A, B and D), aflatoxins, ochratoxin A and other mycotoxins from edible oils. To view this application note in its entirety, click here to view a PDF
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