Bio-Scale™ Mini Profinity™ Cartridges

Comparison of Bio-Scale Mini Profinity Cartridges to Other Affinity Columns on the Profinia™ Protein Purification System

Introduction

Affinity-tagged purification of recombinant proteins is a common procedure in many laboratories. Two commonly used affinity tags are glutathione-S-transferase (GST) and polyhistidine (His). The Profinia protein purification system simplifies the process by automating the purification and desalting of these tagged proteins in a single step.

The Profinia system features:
• Preprogrammed, optimized methods for the most common affinity purifications
• Convenient, prepackaged chromatography buffers and cartridges
• Large, user-friendly touch-screen interface for selecting or customizing methods for specific applications
• Automatic instrument and cartridge cleaning routines, performed after purifications to ensure optimal performance
• Ability to produce similar quality target protein by multiple users

Bio-Scale Mini Profinity cartridges are designed for use on the Profinia system and offer a number of advantages, including ease of use and reproducibility. Here we compare the performance and reproducibility of other commercially available affinity columns to Bio-Scale Mini Profinity cartridges when used on the Profinia protein purification system.

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