A key theory of the cell cycle of asymmetric bacteria, which has prevailed for the last 10 years, has been disproved by a combined approach using mathematical modeling and genetic experiments.
Modelers Prof. Martin Howard and Seán Murray, from the John Innes Centre on the Norwich Research Park, together with experimental collaborators Prof. Patrick Viollier, Gaël Panis and Coralie Fumeaux at the Univ. of Geneva, had been researching how viruses could hijack the natural cell cycle of Caulobacter crescentus, a bacterium found in water.
Caulobacter divides asymmetrically – a stationary “mother” cell divides to produce a motile daughter cell that, unlike the mother, can swim by using a rotating hair-like structure called a flagellum. Unable to reproduce as it is, the daughter cell must first develop into a new mother cell, shedding its flagellum and gaining a stalked appendage that binds it to a surface. This kind of asymmetric life cycle can be seen across nature and is the same type of mechanism used by many multi-cellular organisms like coral.
But while developing and testing simple mathematical models of the Caulobacter cell cycle to study its interaction with viruses, they realized that two of four key cell cycle genes thought to be essential for cell viability, were actually not needed at all. Moreover, these two inessential genes turned out to form a regulatory circuit that was conserved in many other asymmetric bacteria, but which was likely absent from Caulobacter’s original (and simple) evolutionary ancestors.
“The prevailing view was that without all four of these core cell cycle genes, the cells would not develop properly and would die,” says Howard. “Through mathematical modeling, coupled with biological experiments from our colleagues in Geneva, we have shown that prevailing view to be false, as we were able to delete two of the four genes and still obtain viable cells.”
The machinery at the heart of asymmetric cell cycle control is thus far simpler than previously believed. This simplicity, and the combined minimal modeling/genetics approach which revealed it, are potentially important for many other cell cycles in more complex organisms. These results have been published in the high impact journal PLoS Biology.