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Micro-Fabrication Aids Development of Lab-on-a-Chip

Wed, 02/13/2013 - 7:00am
Univ. of Illinois at Urbana-Champaign

Cross-section of device with a droplet. The left side shows an unheated droplet with the DNA FRET construct in the double-stranded form. The right side shows a heated droplet where the FRET construct has denatured, resulting in an increase in fluorescence. Image: Univ. of Illinois at Urbana-ChampaignMiniaturized laboratory-on-chip systems promise rapid, sensitive, and multiplexed detection of biological samples for medical diagnostics, drug discovery, and high-throughput screening. Using micro-fabrication techniques and incorporating a unique design of transistor-based heating, researchers at the Univ. of Illinois at Urbana-Champaign are further advancing the use of silicon transistor and electronics into chemistry and biology for point-of-care diagnostics.

Lab-on-a-chip technologies are attractive as they require fewer reagents, have lower detection limits, allow for parallel analyses and can have a smaller footprint.

“Integration of various laboratory functions onto microchips has been intensely studied for many years,” explains Rashid Bashir, professor of electrical and computer engineering and of bioengineering at Illinois. “Further advances of these technologies require the ability to integrate additional elements, such as the miniaturized heating element, and the ability to integrate heating elements in a massively parallel format compatible with silicon technology. In this work, we demonstrated that we can heat nanoliter volume droplets, individually and in an array, using VLSI silicon based devices, up to temperatures that make it interesting to do various biochemical reactions within these droplets.”

“Our method positions droplets on an array of individual silicon microwave heaters on chip to precisely control the temperature of droplets-in-air, allowing us to perform biochemical reactions, including DNA melting and detection of single base mismatches,” says Eric Salm, first author of the paper published in the Proceedings of the National Academy of Science.

According to Salm, approaches to perform localized heating of these individual subnanoliter droplets can allow for new applications that require parallel, time- and space multiplex reactions on a single integrated circuit. Within miniaturized laboratory-on-chips, static and dynamic droplets of fluids in different immiscible media have been used as individual vessels to perform biochemical reactions and confine the products.

“This technology makes it possible to do cell lysing and nucleic acid amplification reactions within these individual droplets – the droplets are the reaction vessels or cuvettes that can be individually heated,” Salm adds.

“We also demonstrate that ssDNA probe molecules can be placed on heaters in solution, dried, and then rehydrated by ssDNA target molecules in droplets for hybridization and detection,” says Bashir. “This platform enables many applications in droplets including hybridization of low copy number DNA molecules, lysing of single cells, interrogation of ligand–receptor interactions, and rapid temperature cycling for amplification of DNA molecules. Notably, our miniaturized heater could also function as dual heater/sensor elements, as these silicon-on-insulator nanowire or nanoribbon structures have been used to detect DNA, proteins, pH, and pyrophosphates. By using micro-fabrication techniques and incorporating the unique design of transistor-based heating with individual reaction volumes, ‘laboratory-on-a-chip’ technologies can be scaled down to ‘laboratory-on-a-transistor’ technologies as sensor/heater hybrids that could be used for point-of-care diagnostics.”

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