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New Method Speeds Food Safety Tests

Tue, 01/15/2013 - 8:36am
The Univ. of Queensland

Screening for bacteria just got speedier. Image: The Univ. of QueenslandChicken, meat and other foods will be able to be screened for bacteria even faster and more effectively than ever, thanks to breakthrough nanobiotechnology research.

A team of scientists from The Univ. of Queensland (UQ) and the Department of Agriculture, Fisheries and Forestry (DAFF) will leverage this new technology which enables DNA amplification on “microspheres” to rapidly detect and identify large numbers of different bacteria at once.

Prof. Ross Barnard, Director of the Biotechnology Program at the UQ School of Chemistry & Molecular Biosciences, says that authorities have estimated that there are around 5.4 million cases of food-borne gastroenteritis in Australia every year. Of these cases, it is estimated that around 200,000 are associated with the bacteria Campylobacter jejuni and Campylobacter coli.

“We hope to use this new technology to be able to detect and type C. jejuni/coli. These quick identification techniques can underpin relevant and sustainable programs to further improve food safety,” he says. “The infectious dose for C. jejuni/coli can be very low – around 500 organisms. This means that sensitive, specific and rapid techniques are particularly important for this organism.”

He says while testing methods do exist, they had been slow and less effective, so many scientists had turned their focus to leveraging existing “microsphere” technology to a new level.

“After five years, we are now able to extend and develop the platform in ways that haven't been done before,” Barnard says. “We will now be able to carry out many typing reactions at once by doing a very large number of DNA amplification reactions at the same time on the surface of the microspheres.”

The continuing research will be sponsored by the Poultry CRC (Cooperative Research Centre) and carried out by UQ PhD student Liang Fang, who is working on an International Postgraduate Research/UQ Centenary Scholarship, alongside Pat Blackall and Jillian Templeton at DAFF.

The discovery was the result of five years of intensive research and the full scale of the benefits is yet to be known.

“This is just the beginning. Because this testing is based on a platform technology it can be applied in many different ways, such as mutation screening in plant, animal and human genomes, as well as for applications in the realm of infectious diseases,” says Barnard.

The discovery has attracted global interest featuring this month on the front cover of the respected international journal Analytical Biochemistry and has resulted in an invitation to present the work at the Luminex International Diagnostics Forum in Monaco.

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