Using synthetic foam-type materials to mimic the natural process – known as the extracellular matrix or ECM– scientists from the Univ. of Sheffield and Univ. of California San Diego have recreated the random stickiness required for stem cells to properly adhere.
The findings will better inform researchers across the world of how to make their biomaterials appropriately sticky for stem cells to grow.
Previous attempts to recreate the process have managed only a uniform spread of sticky cells meaning there isn’t the maximum hindering the stem cells maturation into tissue cells.
“We used two polymers, one that is sticky and one that is not, which separate from each other in solution,” says Prof. Giuseppe Battaglia of the university’s Department of Biomedical Science. “Just like with balsamic vinaigrette, we shook these two polymers up sufficiently to form randomly distributed nano-scopic patches of the sticky material – the balsamic vinegar –in a non-sticky material, just like the olive oil. To put it another way, these two materials phase separate within the foam to give you regions distinctly of one material or the other.”
At the appropriate ratio of sticky and non-sticky polymer, the researchers found that it is possible to tune the size and distribution of the foam’s adhesive regions: having less sticky polymer in the foam made its adhesive patches smaller and more dispersed, just like in the human body with natural ECM.
“What was surprising to the team was that when we allowed stem cells to adhere to the foams, we found that random stickiness versus uniform stickiness was required for stem cells to properly adhere.,” added Prof. Battaglia and Priyalakshmi Viswanathan, who performed most of the experimental work. “We also found that this is likely necessary for stem cell development into mature tissue cells. In this sense, stem cells are like Goldilocks: the scaffold should not be too sticky or not sticky enough, it must be just right to maximize adhesion, and later, maturation into tissue cells.”
The data was published following a collaboration between Battaglia, and Assistant Prof. of Bioengineering from San Diego Adam Engler in the Journal of the American Chemical Society.