Ultra-High-Speed Separation of Amino Acids

Pre-column derivatization with fluorescence detection

Yasuyo Sato, Craig Silverman, Masao Bounoshita, Toshihiko Miyaji, Keijin. Iwaya, Takayuki Yamaguchi and Muneo Saito

Amino acid analysis is becoming more important in a variety of

Articles

Soy Studies, 10x Faster
Small....and Walking Tall

Products
UPLC Benefits Made Accessible to HPLC Users
LC-MS Series Triples the Sensitivity
HPLC and UHPLC in One Efficient System
LC System Cuts Downtime and Expenditure

News
ExpressLC-Ultra Combines UHPLC Performance with MicroLC
New Zealand Firms Turn Algae Into Biofuel
Waters Ships Chrom Data Software
Spectrum Intros New Solvents for LCMS

application fields, ranging from food analysis to protein science. A number of separation and detection methods are currently used. Among them, a combination of pre-column derivatization with OPA (orthophthalic aldehyde) and separation on a C18 column with fluorescence detection is generally preferred due to the simplicity and high sensitivity of the method.^1-6

One conventional method uses a 4.6-mm I.D. × 150/250-mm L column packed with 5-μm diameter packing material and requires approximately 38 minutes to complete the analysis.

Due to the graphs in this article a PDF has been made available for download.

Company’s Other Products
JASCO Incorporated
28600 Mary's Court
Easton MD 21601
Phone: 410-822-1220
Fax: 410-822-7526
www.jascoinc.com

Type Your Comment...

Name:
E-mail (optional):	*E-mail addresses do not publish to the site.
Comment:

Chrom Notebook

Searching for Cousteau

Tim Studt
Editor in Chief

I often wonder what Jacques Cousteau would say about the state of affairs of the world's oceans. What would he think about the floating islands of plastic garbage in the Pacific twice the size of Texas, or about rapid ocean acidification linked to increasing carbon dioxide levels in the atmosphere, or about changing ocean currents because of melting icecaps and glaciers, or about BP's Deepwater Horizon oil spill in the Gulf of Mexico? ...continue

View the Notebook