Enhanced Detection of Semi-Volatile Compounds using Sub-Ambient ELSD

by Stephen Bullock, Polymer Laboratories, now a part of Varian, Inc.

Evaporative light scattering detection (ELSD) is a powerful tool for detecting any sample that is less volatile than the mobile phase in HPLC applications, irrespective of the optical properties of the compounds of interest. ELSD can therefore offer distinct advantages over the more conventional UV or DAD detection, particularly for gradient separations. Over recent years, the analysis of non-UV absorbing semi-volatile analytes (eg drug candidates) has required ELS detectors to operate at increasingly lower temperatures in order to maximize their detection.

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