by Stephen Bullock, Polymer Laboratories, now a part of Varian, Inc.
Evaporative light scattering detection (ELSD) is a powerful tool for detecting any sample that is less volatile than the mobile phase in HPLC applications, irrespective of the optical properties of the compounds of interest. ELSD can therefore offer distinct advantages over the more conventional UV or DAD detection, particularly for gradient separations. Over recent years, the analysis of non-UV absorbing semi-volatile analytes (eg drug candidates) has required ELS detectors to operate at increasingly lower temperatures in order to maximize their detection.
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Searching for Cousteau
I often wonder what Jacques Cousteau would say about the state of affairs of the world's oceans. What would he think about the floating islands of plastic garbage in the Pacific twice the size of Texas, or about rapid ocean acidification linked to increasing carbon dioxide levels in the atmosphere, or about changing ocean currents because of melting icecaps and glaciers, or about BP's Deepwater Horizon oil spill in the Gulf of Mexico? ...continue