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Expanding SFC Capability
Novel centrifugal fraction collector facilitates SFC-MS purification
William Farrell, Christine Aurigemma, David Masters-Moore and Herbert Hedberg
Supercritical fluid chromatography utilizes a non-polar compressed gas at room temperature, such as carbon dioxide (CO2), and a polar modifier, such as methanol, to elute analytes from the stationary phase. Due to the properties of CO2, diffusivity of the analyte into the stationary phase is increased and the viscosity of the mobile phase is reduced, leading to a lower column pressure drop when compared to HPLC.
These factors are particularly advantageous for high-throughput chromatography and for chiral separations where compound interaction with the stationary phase is increased and separations can be achieved at high flow rates without sacrificing resolution, when compared to the same phase using HPLC.1-5 Coupling SFC with mass spectrometry has been demonstrated for the analysis of combinatorial libraries with subsequent purification using SFC with UV detection.6-8
Since SFC uses a gas under pressure to perform the chromatographic separation, great care must be taken to prevent loss of material upon expansion of the gas during depressurization. Wang et. al. reported average recoveries greater than 77% using a make-up stream of methanol post-nozzle to minimize vaporization and increase recovery.9 Other SFC fraction collectors use pressurized collection containers to minimize and control the mobile phase expansion to achieve recovered yields greater than 90% (Thar Instruments, Inc., Pittsburgh, PA). The prototype CFC-1 used in this report utilizes centrifugal force to collect the droplets of non-volatile aerosol into a collection tube during fraction collection, maximizing recovery without the use of make-up solvent or complex pressurized collection systems.
Due to the graphs in this article a PDF has been made available for download.
Modular SFC. LLC
167 S. Washington Street N.
Attleboro, MA, 02760-2235
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