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Targeted Protein Quantitation Using Triple Quadrupole Mass Spectrometry

Targeted protein quantitation in complex matrices can now be achieved routinely by performing high-throughput highly selective reaction monitoring (H-SRM) analyses using the TSQ Quantum Ultra triple quadrupole mass spectrometer. By reducing matrix interference without significant signal loss, the spectrometer provides enhanced assay sensitivity, selectivity, and robustness, enabling researchers to accurately quantify more proteins more reliably. H-SRM scans are fast (2 milliseconds) and sensitive, allowing practical monitoring and quantitation of hundreds of proteins per second during LC-MS-MS analysis. With this speed, complicated MRM-scheduling methods are no longer needed; proteins are monitored during the entire analysis. In addition, the triple-quadrupole-based method produces confirmation of peptide structure, as targeted proteins are identified by automatic data dependent MS-MS scans.
Thermo Scientific
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Milford, MA, 01757