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Automated SFC Method Development:
Acetaminophen, Benzoic Acid, and Procaine Separation on Pyridine Column Using SuperPure Discovery Analytical SFC Instrument
by Harbaksh Sidhu and Michael Webster, Thar Technologies Inc.
The SuperDiscovery is a new analytical supercritical fluid chromatography system from Thar Technologies. The unit is capable of automated method development or analytical screening of compounds.
System Specification
System Pressure
up to 400 bar
Total System Flow
0.5 to 10 mL/min
Detection
VWD UV/Vis
Fluid Delivery Module
CO2: 0.1 to 10 mL/min
Co-solvent: 0.01 to 10 L/min
Solvent Selection: up to 6
Back Pressure Regulator
0.5 to 10 mL/min, up to 400 bar
Column Oven
5 to 90 C with six-port column selection valve
Autosampler
96-well plate, 48 vials or 384-well plate
Conditions
Total Flow: 4 mL/min Mobile Phase: CO2 + MeOH (13%) Pressure: 100 Bar
Injection: 1 μL ( 1 mg/mL concentration of each in mixture) VWD: 254 nm, 0.020 sensitivity
Peak 1: Benzoic Acid; Peak 2: Procaine; Peak 3: Acetaminophen Column: Pyridine 60A 5u @ 40 deg. C
Thar Technologies, Inc.
100 Beta Drive
Pittsburgh, PA 15238
Thar Instruments, Inc. 575 Epsilon Dr., Suite 100 Pittsburgh, PA, 15238
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