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Automated SFC Method Development:

Acetaminophen, Benzoic Acid, and Procaine Separation on Pyridine Column Using SuperPure Discovery Analytical SFC Instrument

by Harbaksh Sidhu and Michael Webster, Thar Technologies Inc. The SuperDiscovery is a new analytical supercritical fluid chromatography system from Thar Technologies. The unit is capable of automated method development or analytical screening of compounds.


System Specification System Pressure up to 400 bar Total System Flow 0.5 to 10 mL/min Detection VWD UV/Vis Fluid Delivery Module CO2: 0.1 to 10 mL/min Co-solvent: 0.01 to 10 L/min Solvent Selection: up to 6 Back Pressure Regulator 0.5 to 10 mL/min, up to 400 bar Column Oven 5 to 90 C with six-port column selection valve Autosampler 96-well plate, 48 vials or 384-well plate Conditions Total Flow: 4 mL/min     Mobile Phase: CO2 + MeOH (13%)      Pressure: 100 Bar      Injection: 1 μL ( 1 mg/mL concentration of each in mixture)     VWD: 254 nm, 0.020 sensitivity Peak 1: Benzoic Acid; Peak 2: Procaine; Peak 3: Acetaminophen Column: Pyridine 60A 5u @ 40 deg. C Thar Technologies, Inc. 100 Beta Drive Pittsburgh, PA 15238
Thar Instruments, Inc.
575 Epsilon Dr., Suite 100
Pittsburgh, PA, 15238





















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