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Characteristics of an Ultra-Low Solvent Delivery System for Spectroscopic Applications: The Scivex™ Confluent™ Nano Fluidic Module
Darren F. Lewis, Ph.D., Research Scientist
Increasingly, methods are being developed that demand sensitive high-throughput spectroscopic analyses of biologically relevant materials in small quantities. Many of these analyses require precise control of fluid flow rates in the 100-nL to 5-μL/minute range, with pressures ranging over several orders of magnitude. The objective of this application note is to explore some of the low-flow characteristics of the Scivex Confluent Nano Fluidic Module (NFM) when used in analyses that develop significant backpressure (e.g., with an in-line nano column).
Experimental
Figure 1 depicts the experimental setup used to evaluate NFM flow characteristics. Briefly, the NFM consists of three integrated components: a high-pressure positive displacement pump; an inline, non-invasive flow sensor; and a four-way selection valve for filling and dispensing solvent mixtures. The NFM maintains flow rate precision by using the flow sensor signal to adjust pump piston velocity, thus clamping the output flow rate to a selected value, regardless of system backpressure. Downstream from the NFM outlet, an Upchurch® V-485 NanoPeak™ injection valve was used to inject sample plugs onto a 15 cm × 75 μm ID nano column (PepMap™ C18 column, LC Packings, Amsterdam, The Netherlands). Using a timed injection routine, repetitive 5-nL plugs of a mixture consisting of naphthalene, fluorene, biphenyl, and uracil dissolved in 75% acetronitrile/water were injected onto the column. Analytes were detected via absorbance at 250 nm using LC Packings' UltiMate™ UV detector. All experimental data were collected at 1.6 Hz using A/D circuitry and software developed in-house. Column temperature was carefully maintained at 35.0 ±
0.05°C using an in-house constructed column oven.
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Upchurch Scientific
IDEX Health & Science Group
619 Oak Street
Oak Harbor, WA, 98277
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