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Proteome Partitioning Chemistries for Biomarker Discovery

The IgY-R7 rodent and single-component chemistries add to the family of ProteomeLab partitioning systems for the isolation and analysis of protein-based biomarkers. The rodent chemistry selectively partitions seven highly abundant proteins, up to 70% of the protein mass, from rat or mouse serum or plasma. The single-component kits specifically capture a choice of seven key, highly abundant plasma proteins, enabling a more targeted approach for specific protein partitioning. The chemistries are based on avian-generated antibodies and offer a clean capture and broad antigen-binding host range. They produce an enriched proteome plentiful in low-abundant proteins—the primary target for biomarker discovery. This material can then be collected and fractionated by the ProteomeLab PF 2D system or applied to a 2-D electrophoresis gel and further analyzed by mass spectrometry or immunoassay.
Beckman Coulter, Inc.
4300 N. Harbor Blvd., P.O. Box 3100
Fullerton, CA, 92834