Evaluation of an Automated Gel Permeation Chromatography (GPC) System for the Cleanup of Shrimp Prior to Antibiotic Analysis
Introduction
Antibiotics are often used in aquaculture to treat infections in a variety of fish, crawfish, and shrimp. The emergence of bacteria resistant to antimicrobials has caused concern about antibiotic presence in the human food supply. The FDA has recently increased its antibiotic monitoring of seafood products for import into the United States.
There is great interest in developing methods capable of analyzing antibiotics in aquaculture products. Roybal et al. (Annual AOAC Meeting, Sept. 2002) recently described an extraction and cleanup procedure for the analysis of several sulfonamide compounds in shrimp. The authors used Sephadex® LH-20 size exclusion gels to clean shrimp tissue prior to sulfonamide analysis by HPLC. This method calls for multiple manual solvent additions for effective GPC cleanup.
The OI Analytical AutoPrep 2000 System automated GPC cleanup system features an autosampler that injects up to 60 sample extracts, then collects the purified fractions in a variety of vessels after GPC column cleanup. The purpose of this study was to evaluate the possibility of using the AutoPrep 2000 GPC system in combination with an EnviroSep™-ABC column for the cleanup of shrimp prior to antibiotic analysis.
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