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Quantitative Determination of Nonionic Surfactants with CAD
A challenge is simplified for pharmaceutical manufacturers
Carmen Staar
Nonionic surfactants such as Tween 80 (Polysorbate 80) and Span 85 (sorbitan trioleate) are widely used in pharmaceutical formulations as multipliers, wetting agents and dosing vehicles. Regulatory authorities are increasingly asking for methods to quantify the amount of residual surfactants and other ingredients in the final product. Nonionic surfactants are not charged, nor do they have a chromophore. In the past this has meant that a time-consuming derivatization of the analytes was necessary to implement charge and ultraviolet (UV) activity for high-performance liquid chromatography (HPLC) with UV detection. In this present study, HPLC and ultra-performance liquid chromatography (UPLC) in combination with an evaporative light scattering detector (ELSD) and a charged aerosol detector (CAD) were successfully used to quantify Tween 80 and Span 85. ELSD and CAD each provide a universal detection method capable of detecting any non-volatile analyte. CAD offers a 10-fold advantage in sensitivity, as well as greater linearity.
Due to the graphs in this article a PDF has been made available for download.
ESA Biosciences Inc.
22 Alpha Road
Chelmsford, MA, 01824-4171
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